Preparation and cytocompatibility of novel porous calcium metaphosphate composite membranes

doi:10.3969/j.issn.2095-4344.2013.34.010

Abstract

Abstract:

BACKGROUND: Porous poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes prepared previously is too thick and uneven in holes.

OBJECTIVE: To prepare the thin even porous poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membrane, and to evaluate the cytocompatibility and differentiation capacity.

METHODS: Porous and nonporous, thin and even poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes were prepared by phase separation method. Its thickness and weight loss rate were determined. Human bone marrow mesenchymal stem cells were cocultured with porous and nonporous poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes for 7 days. Ultrastructure of composite membranes was observed under the scanning electron microscopy. Surface markers of the bone marrow mesenchymal stem cells on the composite membranes were analyzed using flow cytometry.

RESULTS AND CONCLUSION:The thickness of the porous and nonporous composite membranes was (0.041 ± 0.005) mm and (0.058±0.004) mm. Weight loss rates of porous and nonporous composite membranes were respectively 19.93% and 7.64% at 24 hours. Calcium metaphosphate particles were evenly distributed in porous and nonporous composite membrane. Cells spread entirely, showing spindle shape. Calcium metaphosphate particles were evenly distributed in porous composite membrane. Pore in porous composite membranes was also uniformly distributed, and pore size was about 2-8 μm. Cells spread entirely, showing polygonal shape with multiple tentacles. The tentacles of some cells entered into the scaffold. CD105, CD90, CD44, CD29 and CD73 expression was detected in porous and nonporous composite membranes. There was no significant difference in cell-positive rate. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate composite membranes prepared in this study has good biocompatibility and could not promote cell differentiation.

Key words: biomaterials, material biocompatibility, membrane biomaterial, composite membranes, poly(3-hydroxybutyrate-co-4-hydroxybutyrate, calcium metaphosphate, porous, human bone marrow stromal stem cells, National Natural Science Foundation of China

CLC Number:

R318

Wu Yue-heng, Chen Peng, Mai Li-ping, Zhang Ling-min, Tang Shun-qing, Yu Xi-yong. Preparation and cytocompatibility of novel porous calcium metaphosphate composite membranes[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6123-6130.

Figures/Tables 4

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